Caspase 3 Activity Assay Kit

Reference: P40120

Caspase-3 is a member of caspases that plays a key role in mediating apoptosis, or programmed cell death. Upon activation, it cleaves a variety of cellular proteins, causing morphological and functional changes to cells undergoing apoptosis.

Assay Characteristics

The Innoprot Caspase-3 Activity Assay provides a quick and convenient method to measure caspase-3 activity. The colorimetric assay is based on the spectrophotometric detection of the chromophore p-nitroanilide (pNA) after its cleavage by caspases-3 from the labeled substrate acetyl-Asp-glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA). The concentration of pNA is measured by absorbance at 405 nm. The caspase-3 activity can be calculated as µmol of pNA released per minute per milliliter of cell lysate.

Kit Components

Reagents for 100 Tests

Reagent	Quantity	Storage
Lysis Buffer	10 ml	2 - 8°C
DTT Stock (1 M)	0.2 ml	- 20°C
Assay Buffer 1X	3 ml	2 - 8°C
Caspase 3 Substrate (2mM)	1 ml	- 20°C, dark
pNA Standard (40 mM)	20 ul	- 20°C

Caspase 3 Activity Assay Kit

Assay Characteristics

Kit Components

Related Products/Services