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Innoprot

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Primary Cells Detach Kit

Reference: P60305


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Data Sheet

Primary Cells Detach Kit from Innoprot contains reagents for primary cells detach and subculture.

Kit Components

  • 500 ml DPBS (Dulbecco’s Phosphate-Buffered Saline)
  • 100 ml T/E Solution (Trysin / EDTA solution)
  • 100 ml TNS (Trysin Neutralization Solution)

Components Description


Dulbecco’s Phosphate-Buffered Saline is a commonly used buffer in biological research. DPBS helps maintain a constant pH. The solution does not conatin Ca2+ or Mg2+ and is sterile-filtered. Because it is not toxic to cells and is isotonic, it may be used to rinse cells without causing any damage to them.

Trysin / EDTA solution (T/E) is a sterile, phosphate and HEPES-buffered saline solution. It contains 0,25% trypsin, 0,5 mM EDTA, 1 mM sodium pyruvate and 10 mM HEPES. This product has a pH of 7.4 at room temperature. Trypsyn/EDTA Solution is used to detach adherent cells from a culture surface.

Trysin Neutralization Solution (TNS) is a sterile, phosphate and HEPES-buffered saline solution. It contains 10% fetal bovine serum as a trypsin inhibitor and cell protect agents. The product is calcium- and magnesium-free and has a pH of 7.4 at room temperature. Trypsyn Neutralization Solution is used to neutralize the effects of Trypsin/EDTA solution after the release of cells from a culture surface.
 

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